Cellufine™ MAX IEX chromatography media are optimized for high adsorption and resolution. The graphs below show the separation of model proteins. Good separation profiles are observed.
Cellufine™ MAX IEX chromatography media maintain high binding capacity over a range of salt concentrations. This characteristic enables the omission of buffer exchange steps prior to the use of Cellufine™ chromatography media.
The binding capacity of ion exchange chromatography media depends on ionic strength and pH. Separation conditions need to be optimized depending on the characteristics of the target proteins.
The graphs below show that Cellufine™ MAX S-h media has a higher IgG binding capacity than Agarose S type media with 10 mM NaCl at all pH levels tested. Even with 100 mM NaCl, Cellufine™ MAX S-h media demonstrates higher IgG DBC at pH 4.3.