Cellufine™ IEX

Excellent adsorption & resolution

Cellufine™ MAX IEX chromatography media are optimized for high adsorption and resolution. The graphs below show the separation of model proteins. Good separation profiles are observed.

Cellufine™ MAX Q-h & Cellufine™ MAX DEAE

Cellufine™ MAX Q-h & Cellufine™ MAX DEAE Graph
Column:
6.6 mm I.D. x 50 mm
Equilibration buffer (A):
50 mM Tris-HCl (pH 8.5)
Elution buffer (B):
1 M NaCl in (A)
Elution:
(B) 0% → (B) 75%, linear gradient
Flow rate:
0.86 mL/min (residence time 2 min)
Sample loaded:
1.5 mL
Transferrin (5 mg/mL)
BSA (10 mg/mL)
Pepsin (5 mg/mL)

Cellufine™ MAX S-h & Cellufine™ MAX CM

Column:
6.6 mm I.D. x 50 mm
Equilibration buffer (A):
10 mM phosphate (pH 7)
Elution buffer (B):
1 M NaCl in (A)
Elution:
(B) 0% → (B) 50%, linear gradient
Flow rate:
0.86 mL/min (residence time 2 min)
Sample loaded:
1.5 mL
Ribonuclease A (5 mg/mL)
Cytochrome C (2.5 mg/mL)
Lysozyme (1.5 mg/mL)

High salt tolerance

Cellufine™ MAX IEX chromatography media maintain high binding capacity over a range of salt concentrations. This characteristic enables the omission of buffer exchange steps prior to the use of Cellufine™ chromatography media.

BSA DBC at 10% Breakthrough for Various NaCl Concentrations

BSA DBC at 10% Breakthrough for Various NaCl Concentrations Graph
BSA concentration:
1 mg/mL
Column:
5 mm I.D. x 100 mm
Buffer:
NaCl in 50 mM Tris-HCl (pH 8.5)
Flow rate:
300 cm/h (residence time 2 min)

IgG DBC at 10% Breakthrough for Various NaCl Concentrations

IgG concentration:
1 mg/mL (polyclonal, human)
Column:
5 mm I.D. x 100 mm
Buffer:
NaCl in 10 mM Na-acetate (pH 4.3)
Flow rate:
300 cm/h (residence time 2 min)

Optimization of binding conditions

The binding capacity of ion exchange chromatography media depends on ionic strength and pH. Separation conditions need to be optimized depending on the characteristics of the target proteins.

The graphs below show that Cellufine™ MAX S-h media has a higher IgG binding capacity than Agarose S type media with 10 mM NaCl at all pH levels tested. Even with 100 mM NaCl, Cellufine™ MAX S-h media demonstrates higher IgG DBC at pH 4.3.

IgG DBC at 10% Breakthrough for Various Salt Concentrations and pH Levels

Cellufine™ MAX S-h

Cellufine™ MAX S-h Graph
IgG concentration:
1 mg/mL (polyclonal, human)
Column:
5 mm I.D. x 100 mm
Buffer:
NaCl in 10 mM Na-acetate
Flow rate:
300 cm/h (residence time 2 min)

Agarose S Type Media

IgG concentration:
1 mg/mL (polyclonal, human)
Column:
5 mm I.D. x 100 mm
Buffer:
NaCl in 10 mM Na-acetate
Flow rate:
300 cm/h (residence time 2 min)
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