Cellufine™ Affinity

Cellufine™ ET Clean Media

Cellufine™ ET Clean media binds and removes endotoxins from protein solutions. The poly(ε-lysine) ligand, which is immobilized on chloromethyloxirane-activated cellulose beads, is a microbial poly(amino acid) that consists of 30 to 35 lysine residues produced by Streptomyces albulus. The media shows good tolerability to cleaning solutions, which include 0.2 M NaOH and 2 M NaCl.

Cellufine™ ET Clean Media Image

Cellufine™ Chelate Media

Cellufine™ Chelate media is designed for use in immobilized metal chelate affinity chromatography of proteins and peptides. The media comprises cross-linked cellulose to which iminodiacetic acid (IDA) is immobilized. Its rigid structure allows operating under high flow rates, which gives rapid processing times. When exposed to metal salts, IDA readily complexes with the cation. The resulting metal chelate moiety interacts (primarily) with the surface accessible residues, such as histidine tag.

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